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Image Search Results
Journal: eLife
Article Title: In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins
doi: 10.7554/eLife.101967
Figure Lengend Snippet:
Article Snippet: Commercial assay or kit ,
Techniques: Recombinant, Plasmid Preparation, Magnetic Beads, Transfection, Western Blot, Software
Journal: Journal of Virology
Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
doi: 10.1128/jvi.00057-24
Figure Lengend Snippet: (A) Immunoprecipitation of 293T lysate expressing HA-ZCCHC14 with purified recombinant FLAG-TENT4A or FLAG-TENT4B proteins. BSA is used as a negative control. (B) IP as in (A) with indicated ZCCHC14 constructs. (C) IP as in (B) with RNase 1 treatment. (D) (left) Diagram depicting deletions made in the Z14-N fragment and (right) their interaction with TENT4 in the co-IP assay. (E) (left) Diagram depicting deletions made in the Z14-C fragment and (right) their interaction with TENT4 in the co-IP assay.
Article Snippet: For this purpose, 293T lysates expressing HA-tagged ZCCHC14 fragments were incubated with purified recombinant FLAG-tagged TENT4 protein fragments (aa 226–558 of TENT4A or aa 186–518 of TENT4B) representing the catalytic domains of these noncanonical polymerases (kindly provided by
Techniques: Immunoprecipitation, Expressing, Purification, Recombinant, Negative Control, Construct, Co-Immunoprecipitation Assay